Phosphatase Family CDC25 - Revision history

Mark: /* Acr2 */

2015-11-20T00:45:41Z

‎Acr2

Mark: /* CDC25/YCH1 */

2015-11-20T00:44:13Z

‎CDC25/YCH1

Mark: /* Acr2 */

2015-11-16T19:03:23Z

‎Acr2

Mark at 06:35, 16 November 2015

2015-11-16T06:35:37Z

Gerard at 01:47, 23 August 2015

2015-08-23T01:47:38Z

Gerard at 01:29, 23 August 2015

2015-08-23T01:29:58Z

Gerard at 23:04, 22 August 2015

2015-08-22T23:04:42Z

Mark at 23:23, 31 December 2014

2014-12-31T23:23:04Z

Mark at 23:22, 31 December 2014

2014-12-31T23:22:52Z

Mark at 23:13, 31 December 2014

2014-12-31T23:13:47Z

@@ Line 18: / Line 18: @@
 Note: We have the same SS annotation of CDC25 in the papers reported CDC25 structures <cite> Fauman98, Reynolds99 </cite>, but we have the SS annotation of YCH1 different from the paper reported its structure <cite>Yeo09</cite>, where it had a SS elements of EHEEHHEHEH. We obtained the original annotations from DSSP and Stride programs and validated them in structure visualization.
-==== Acr2 ====
+==== Compare the structures of human CDC25s and Arabidopsis  Acr2 ====
 Arabidopsis Acr2 has a solution NMR structure (PDB [http://www.rcsb.org/pdb/explore/explore.do?structureId=1T3K 1T3K]). It lacks H1, E3 and E7. It also has slight difference in H3 localization.

@@ Line 7: / Line 7: @@
 === Phosphatase domain structure ===
-==== CDC25/YCH1 ====
+==== Compare the structures of CDC25 and Acr2 subfamilies ====
 The three human CDC25s have generally the same structure, which have a secondary structure (SS) combination of E1, H1, E2, H2, E3, E4, H3, E5, H4, E6, H5, E7. The strands E1, E2, E4, E5, E6 form a 5-stranded sheet; the strands E3 and E7 form a 2-stranded sheet.

@@ Line 17: / Line 17: @@
 ==== Acr2 ====
-See PDB [http://www.rcsb.org/pdb/explore/explore.do?structureId=1T3K 1T3K].
+Arabidopsis Acr2 has a solution NMR structure (PDB [http://www.rcsb.org/pdb/explore/explore.do?structureId=1T3K 1T3K]). It lacks H1, E3 and E7. It also has slight difference in H3 localization.
 === References ===

← Older revision		Revision as of 06:35, 16 November 2015
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	CDC25 and Acr2 are found in a largely exclusive clades. CDC25 is found in most animals, but is absent from plants, while Acr2 is found in plants but not animals. Most fungi and many protists have both.		CDC25 and Acr2 are found in a largely exclusive clades. CDC25 is found in most animals, but is absent from plants, while Acr2 is found in plants but not animals. Most fungi and many protists have both.
		+
		+	=== Phosphatase domain structure ===
		+	==== CDC25/YCH1 ====
		+	The three human CDC25s have generally the same structure, which have a secondary structure (SS) combination of E1, H1, E2, H2, E3, E4, H3, E5, H4, E6, H5, E7. The strands E1, E2, E4, E5, E6 form a 5-stranded sheet; the strands E3 and E7 form a 2-stranded sheet.
		+
		+	The human CDC25s and yeast YCH1 share the SS elements of E1, H1, E2, E3, E4, H3, E5, H4, E6, H5, E7. But, yeast YCH1 structure is different from human CDC25s in two aspects:
		+	* While human CDC25s have an helix (H2) between E2 and E3, yeast YCH1 has an helix between E4 and H3. We named the SS elements as H2-YCH1.
		+	* Yeast YCH1 has a helix between H4 and E6. We name it as H4'-YCH1.
		+
		+	Note: We have the same SS annotation of CDC25 in the papers reported CDC25 structures <cite> Fauman98, Reynolds99 </cite>, but we have the SS annotation of YCH1 different from the paper reported its structure <cite>Yeo09</cite>, where it had a SS elements of EHEEHHEHEH. We obtained the original annotations from DSSP and Stride programs and validated them in structure visualization.
		+
		+	==== Acr2 ====
		+	See PDB [http://www.rcsb.org/pdb/explore/explore.do?structureId=1T3K 1T3K].
		+
		+	=== References ===
		+	<biblio>
		+	#Fauman98 pmid=9604936
		+	#Reynolds99 pmid=10543950
		+	#Yeo09 pmid=19382206
		+	</biblio>

@@ Line 1: / Line 1: @@
 __NOTOC__
-[[Phosphatase classification|Phosphatase Classification]]: [[Phosphatase_Fold_Rhodanese|Fold CC3 (Rhondanese)]]: [[Phosphatase_Superfamily_CC3|Superfamily CC3 (Rhondanese)]]: [[Phosphatase_Family_CDC25|Family CDC25]]
+[[Phosphatase classification|Phosphatase Classification]]: [[Phosphatase_Fold_Rhodanese|Fold CC3 (Rhodanese)]]: [[Phosphatase_Superfamily_CC3|Superfamily CC3 (Rhodanese)]]: [[Phosphatase_Family_CDC25|Family CDC25]]
 CDC25 consists of two subfamilies, '''[[Phosphatase_Subfamily_CDC25|CDC25]]''' and '''[[Phosphatase_Subfamily_Acr2|Acr2]]'''. CDC25 is is an important phosphatase of cyclin-dependent kinases ([http://kinase.com/wiki/index.php/Kinase_Family_CDK CDKs]), the function role of Acr2 is uncertain: it has arsenate reductase activity but may also be a protein phosphatase.
 CDC25 and Acr2 are found in a largely exclusive clades. CDC25 is found in most animals, but is absent from plants, while Acr2 is found in plants but not animals. Most fungi and many protists have both.

@@ Line 2: / Line 2: @@
 [[Phosphatase classification|Phosphatase Classification]]: [[Phosphatase_Fold_Rhodanese|Fold CC3 (Rhondanese)]]: [[Phosphatase_Superfamily_CC3|Superfamily CC3 (Rhondanese)]]: [[Phosphatase_Family_CDC25|Family CDC25]]
-CDC25 is the only CC2 superfamily family with protein phosphatase activity. It consists of two subfamilies, '''[[Phosphatase_Subfamily_CDC25|CDC25]]''' and '''[[Phosphatase_Subfamily_Acr2|Acr2]]'''. CDC25 is is an important phosphatase of cyclin-dependent kinases ([http://kinase.com/wiki/index.php/Kinase_Family_CDK CDKs]), the function role of Acr2 is controversial.
+CDC25 is the only CC3 superfamily family with protein phosphatase activity. It consists of two subfamilies, '''[[Phosphatase_Subfamily_CDC25|CDC25]]''' and '''[[Phosphatase_Subfamily_Acr2|Acr2]]'''. CDC25 is is an important phosphatase of cyclin-dependent kinases ([http://kinase.com/wiki/index.php/Kinase_Family_CDK CDKs]), the function role of Acr2 is uncertain: it has arsenate reductase activity but may also be a protein phosphatase.
-CDC25 and Acr2 are found in a mostly mutually exclusive manner across eukaryotes. CDC25 is found in most animals, but is absent from land plants and green alga ''Chlamydomonas''. On the other hand, Acr2 is found in most plants and Chalmydomonas, but is absent from animals and green alga ''Ostreococcus''. However, in fungi and amoebozoans, both CDC25 and Acr2 are present.
+CDC25 and Acr2 are found in a largely exclusive clades. CDC25 is found in most animals, but is absent from plants, while Acr2 is found in plants but not animals. Most fungi and many protists have both.