Phosphatase Subfamily ILKAP - Revision history

Gerard: /* Evolution */

2017-07-24T04:21:29Z

‎Evolution

2017-07-24T04:19:21Z

‎ILKAP binds to Palladin

2017-07-24T04:18:38Z

2017-07-23T21:34:09Z

‎ILKAP dephosphorylates ERK and RSK2

2017-07-23T21:33:33Z

‎ILKAP dephosphorylates ERK and RSK2

2017-07-23T21:33:19Z

‎ILKAP interacts with ILK and indirectly modules GSK3β Ser-9 phosphorylation

2017-07-23T21:32:00Z

‎Functions

2017-07-23T21:31:40Z

‎Domain

2017-07-23T18:53:11Z

‎ILKAP interacts with ILK and indirectly modules GSK3β Ser-9 phosphorylation

2015-06-07T23:14:19Z

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 === Evolution  ===
-The ILKAP subfamily emerged in [[Phosphatase_Glossary#Holozoa|holozoa]] and was lost in arthropods. In contrast, its physical interacting partners or substrates, ILK, RSK2 and Palladin, are present in arthropods.
+The ILKAP subfamily emerged in [[Phosphatase_Glossary#Holozoa|holozoa]] and was lost in arthropods. In contrast, its physical interacting partners or substrates, ILK and RSK are present in arthropods.
 === Domain ===

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 ILKAP binds to [http://en.wikipedia.org/wiki/Palladin Palladin] (gene symbol: PALLD), an actin-associated scaffold protein involved in the formation of actin-associated protein complexes, localized in stress fibers, focal adhensions, growth cones and etc. The interaction is mediated by Palladin's last three Ig domains (710-1106) and ILKAP's phosphatase domain (108-392) <cite>Zhou11</cite>. Notice: do not confuse [http://en.wikipedia.org/wiki/Palladin Palladin] with [[Phosphatase_Subfamily_Paladin|Paladin]], which is a pseudophosphatase.
-Palladin emerged in chordates and duplicated in vertebrates, which resulted in two members in human genome, Palladin and Myopalladin ([http://resdev.gene.com/gOrtholog/view/cluster/MC0004711/overview internation database]).
+Palladin emerged in chordates and duplicated in vertebrates, which resulted in two members in human genome, Palladin and Myopalladin ([http://resdev.gene.com/gOrtholog/view/cluster/MC0004711/overview internal database]).
 === References ===

← Older revision		Revision as of 04:18, 24 July 2017
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		+	__NOTOC__
	[[Phosphatase classification\|Phosphatase Classification]]: [[Phosphatase_Fold_PPM\|Fold PPM (PP2C)]]: [[Phosphatase_Superfamily_PPM\|Superfamily PPM (PP2C)]]: [[Phosphatase_Family_PPM\|Family PPM (PP2C)]]: [[Phosphatase_Subfamily_ILKAP\|Subfamily ILKAP]]		[[Phosphatase classification\|Phosphatase Classification]]: [[Phosphatase_Fold_PPM\|Fold PPM (PP2C)]]: [[Phosphatase_Superfamily_PPM\|Superfamily PPM (PP2C)]]: [[Phosphatase_Family_PPM\|Family PPM (PP2C)]]: [[Phosphatase_Subfamily_ILKAP\|Subfamily ILKAP]]

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 ILKAP also associated with other kinases of AGC group, such as other RSKs, MSK1 and MSK2, p70S6K, and PDK1. ILKAP also dephosphorylates ERK at Thr-315 and Thr-333 in the catalytic domain <cite>Doehn04</cite>.
-RSKs emerged in holozoa; but in contrast with ILKAP, it is present in arthropods (see [http://kinase.com/web/current/kinbase/genes/Subfamily/RSKp90/ RSKp90 subfamily in KinBase]).
+RSK emerged in holozoa; but in contrast with ILKAP, it is present in arthropods (see [http://kinase.com/web/current/kinbase/genes/Subfamily/RSKp90/ RSKp90 subfamily in KinBase]).
 ===== ILKAP binds to Palladin =====

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 ILKAP also associated with other kinases of AGC group, such as other RSKs, MSK1 and MSK2, p70S6K, and PDK1. ILKAP also dephosphorylates ERK at Thr-315 and Thr-333 in the catalytic domain <cite>Doehn04</cite>.
-RSKs emerged in holozoa; but in contrast with ILKAP, it is present in arthropods (see [http://kinase.com/web/current/kinbase/genes/Subfamily/RSKp90/ RSKp90 subfamily in KinBase].
+RSKs emerged in holozoa; but in contrast with ILKAP, it is present in arthropods (see [http://kinase.com/web/current/kinbase/genes/Subfamily/RSKp90/ RSKp90 subfamily in KinBase]).
 ===== ILKAP binds to Palladin =====

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 Human ILKAP associated with integrin-linked kinase (ILK) in a manner independent of their catalytic activies <cite>LeungHagesteijn01</cite>. Catalytically active but not mutant ILKAP (H154D) strongly inhibited ILK's substrate, glycogen synthase kinase 3 β (GSK3β), at Ser-9, but did not affect the phosphorylation of another substrate, protein kinase B (PKB/AKT) at Ser-473. However, ILKAP may not directly dephosphorylate Ser-9 on GSK3β <cite>LeungHagesteijn01, Kumar04</cite>. A model was proposed in <cite>Kumar04</cite> (figure 7), in which ILKAP, localized in the cytoplasm, inhibits ILK, promotes GSK3β-dependent Thr-286 phosphorylation, nuclear export and proteolysis of cyclin D1.
-ILKs emerged in holozoa; but in contrast with ILKAP, it is present in arthropods (see [http://kinase.com/web/current/kinbase/genes/Subfamily/ILK/ ILK subfamily in KinBase].
+ILK and ILKAP emerged in holozoa; however, ILK is retained in arthropods, while ILKAP is lost (see [http://kinase.com/web/current/kinbase/genes/Subfamily/ILK/ ILK subfamily in KinBase].
 ===== ILKAP dephosphorylates ERK and RSK2 =====

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 The ILKAP gene is widely expressed across different tissues as shown by Northern blot analysis in rat <cite>Tong98</cite> and RNA-seq data from [http://www.gtexportal.org/home/gene/ILKAP GTEx] in human.
-Human ILKAP is localized to nuclear by interacting with importin through a conserved region from 71-86, particularly Lys-78 and Arg-79 are critical <cite>Zhou13</cite>. It is also localized to cytoplasm and focal adhension, as implied by its interaction with ILK <cite>LeungHagesteijn01</cite>.
+Human ILKAP is localized to nuclei by interacting with importin through a conserved region from 71-86, particularly Lys-78 and Arg-79 are critical <cite>Zhou13</cite>. It is also localized to cytoplasm and focal adhension, as implied by its interaction with ILK <cite>LeungHagesteijn01</cite>.
 ===== ILKAP interacts with ILK and indirectly modules GSK3β Ser-9 phosphorylation =====

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 === Domain ===
-The ILKAP subfamlily has a phosphatase domain and an N-terminal region binding to importin which localizes ILKAP to nuclear <cite>Zhou13</cite>.
+The ILKAP subfamlily has a phosphatase domain and an N-terminal region binding to importin which localizes ILKAP to nuclei <cite>Zhou13</cite>.
 === Functions ===

← Older revision		Revision as of 23:14, 7 June 2015
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−	[[Phosphatase classification\|Phosphatase Classification]]: [[Phosphatase_Fold_PPM\|Fold PPM (PP2C)]]: [[Phosphatase_Superfamily_PPM\|Superfamily PPM (PP2C)]]: [[Phosphatase_Family_PPM\|Family PPM (PP2C)]]: [[Phosphatase_Subfamily_ILKAP\|Subfamily ILKAP]] ~~(PP2Cδ)~~	+	[[Phosphatase classification\|Phosphatase Classification]]: [[Phosphatase_Fold_PPM\|Fold PPM (PP2C)]]: [[Phosphatase_Superfamily_PPM\|Superfamily PPM (PP2C)]]: [[Phosphatase_Family_PPM\|Family PPM (PP2C)]]: [[Phosphatase_Subfamily_ILKAP\|Subfamily ILKAP]]

	=== Evolution ===		=== Evolution ===