Phosphatase Subfamily PTC7 - Revision history

Gerard at 20:35, 11 November 2016

2016-11-11T20:35:56Z

Gerard: /* Technical notes */

2016-11-11T01:04:19Z

‎Technical notes

Gerard: /* Technical notes */

2016-11-11T00:49:52Z

‎Technical notes

Gerard at 21:35, 20 November 2015

2015-11-20T21:35:13Z

Gerard: /* Functions */

2015-11-20T21:16:11Z

‎Functions

Gerard: /* References */

2015-11-20T21:14:19Z

‎References

Gerard: /* Functions */

2015-11-20T21:13:57Z

‎Functions

Gerard: /* Subcellular localization */

2015-11-20T20:52:28Z

‎Subcellular localization

Gerard: /* Subcellular localization */

2015-11-20T20:51:56Z

‎Subcellular localization

Gerard: /* Subcellular localization */

2015-11-20T20:50:45Z

‎Subcellular localization

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-[[Phosphatase classification|Phosphatase Classification]]: [[Phosphatase_Fold_PPM|Fold PPM (PP2C)]]: [[Phosphatase_Superfamily_PPM|Superfamily PPM (PP2C)]]: [[Phosphatase_Family_PPM|Family PPM (PP2C)]]: [[Phosphatase_Subfamily_PTC7|Subfamily PTC7]]
+__NOTOC__
 === Evolution ===
-The PTC7 subfamily is found throughout eukaryotes. It is single copy in most [[Phosphatase_Glossary#Opisthokont|opisthokont]]s.
+PTC7 is found throughout eukaryotes. It is single copy in most [[Phosphatase_Glossary#Opisthokont|opisthokont]]s. It is most closely related to bacterial SpoIIE phosphatases.
 === Domain Architecture===

@@ Line 28: / Line 28: @@
 The combination of PLAT and PPM domain is found in sparsely, by BLASTing monosiga PTC7, both full sequence and the region containing PLAT and PPM, against eukaryotes NR dataset. domain (1-400). Below are the organisms and their genes that have the domain combination of PLAT and PPM:
 * Choanoflagellate: Salpingoeca rosetta (XP_004994085.1)
-* Algae: Monoraphidium neglectum (KIY99478.1), Micromonas sp. RCC299 (XP_002499745.1), Bathycoccus prasinos (XP_007509274.1), Ostreococcus tauri (XP_003078617.1).
+* Algae: Monoraphidium neglectum (KIY99478.1), Micromonas sp. RCC299 (XP_002499745.1), Bathycoccus prasinos (XP_007509274.1), Ostreococcus tauri (XP_003078617.1), Auxenochlorella protothecoides (XP_011395907.1)
 * Cryptomonad: Guillardia theta (XP_005819750.1)
 * Fungus: Blastocystis hominis (CBK25361.2)

@@ Line 27: / Line 27: @@
 ===== Accessory PLAT domain is found in a small number of single cellular eukaryotes =====
 The combination of PLAT and PPM domain is found in sparsely, by BLASTing monosiga PTC7, both full sequence and the region containing PLAT and PPM, against eukaryotes NR dataset. domain (1-400). Below are the organisms and their genes that have the domain combination of PLAT and PPM:
-* Algae: Monoraphidium neglectum (KIY99478.1), Micromonas sp. RCC299 (XP_002499745.1), Bathycoccus prasinos (XP_007509274.1)
+* Choanoflagellate: Salpingoeca rosetta (XP_004994085.1)
-* Eudicot: Capsella rubella (XP_006283668.1)
+* Algae: Monoraphidium neglectum (KIY99478.1), Micromonas sp. RCC299 (XP_002499745.1), Bathycoccus prasinos (XP_007509274.1), Ostreococcus tauri (XP_003078617.1).
 * Cryptomonad: Guillardia theta (XP_005819750.1)
 * Fungus: Blastocystis hominis (CBK25361.2)

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 === Functions ===
-Yeast Ptc7p, dephosphorylates and activates the mitochondrial hydroxylase Coq7p (Cat5p) and so activates coenzyme Q6 biosynthesis.  Ptc7 may reverse the action of the Coq8 kinase (ABC1/YGL119W) (see, e.g. <cite>He</cite>), and in humans, ABC1 kinases ADCK3 and ACDK4 have been implicated in the related coenzyme Q10 biosynthesis <cite>Ashraf</cite>. COQ7 is found in most eukaryotes, except plants and excavates <cite>Martin-Montalvo13</cite>. COQ7 in humans is targeted to mitochondria, cleaved to become trapped in these organelles, and then function in the biosynthesis of ubiquinone, a cofactor in the electron transport chain. It is suggested that S114 in human COQ7 is phosphorylated and controls its activity <cite>Busso</cite>. The uncleaved form of these proteins in the nucleus <cite>Monaghan15</cite>.
+Yeast Ptc7p dephosphorylates and activates the mitochondrial hydroxylase Coq7p (Cat5p) and so activates coenzyme Q6 biosynthesis. Ptc7 may reverse the action of the Coq8 kinase (ABC1/YGL119W) (see, e.g. <cite>He</cite>), and in humans, ABC1 kinases ADCK3 and ACDK4 have been implicated in the related coenzyme Q10 biosynthesis <cite>Ashraf</cite>. COQ7 is found in most eukaryotes, except plants and excavates <cite>Martin-Montalvo13</cite>. Human COQ7 is also mitochondrial and involved in coenzyme Q10 biosynthesis. It is suggested that the conserved S114 residue in yeast COQ7 is phosphorylated and controls its activity <cite>Busso</cite>, though other studies claim that Coq7p is only phosphorylated on 3 residues in the unconserved N-terminal region <cite>Martin-Montalvo11</cite>.
 === Subcellular localization ===
@@ Line 16: / Line 16: @@
 <biblio>
 #Ashraf pmid=24270420
 #He pmid=24406904
 #Lanning pmid=24767988
 #Martin-Montalvo13 pmid=23940037
 #Monaghan15 pmid=25961505
 </biblio>

@@ Line 8: / Line 8: @@
 === Functions ===
-Yeast Ptc7p, dephosphorylates and activates the mitochondrial hydroxylase Coq7p (Cat5p) and so activates coenzyme Q6 biosynthesis.  Ptc7 may reverse the action of the Coq8 kinase (ABC1/YGL119W) (see, e.g. <cite>He</cite>), and in humans, ABC1 kinases ADCK3 and ACDK4 have been implicated in the related coenzyme Q10 biosynthesis <cite>Ashraf</cite>. COQ7 is found in most eukaryotes, except plants and excavates <cite>Martin-Montalvo13</cite>. COQ7 in humans is targeted to mitochondria, cleaved to become trapped in these organelles, and then function in the biosynthesis of ubiquinone, a cofactor in the electron transport chain. The uncleaved form of these proteins in the nucleus <cite>Monaghan15</cite>.
+Yeast Ptc7p, dephosphorylates and activates the mitochondrial hydroxylase Coq7p (Cat5p) and so activates coenzyme Q6 biosynthesis.  Ptc7 may reverse the action of the Coq8 kinase (ABC1/YGL119W) (see, e.g. <cite>He</cite>), and in humans, ABC1 kinases ADCK3 and ACDK4 have been implicated in the related coenzyme Q10 biosynthesis <cite>Ashraf</cite>. COQ7 is found in most eukaryotes, except plants and excavates <cite>Martin-Montalvo13</cite>. COQ7 in humans is targeted to mitochondria, cleaved to become trapped in these organelles, and then function in the biosynthesis of ubiquinone, a cofactor in the electron transport chain. It is suggested that S114 in human COQ7 is phosphorylated and controls its activity <cite>Busso</cite>. The uncleaved form of these proteins in the nucleus <cite>Monaghan15</cite>.
 === Subcellular localization ===

@@ Line 10: / Line 10: @@
 Yeast Ptc7p, dephosphorylates and activates the mitochondrial hydroxylase Coq7p (Cat5p) and so activates coenzyme Q6 biosynthesis.  Ptc7 may reverse the action of the Coq8 kinase (ABC1/YGL119W) (see, e.g. <cite>He</cite>). COQ7 is found in most eukaryotes, except plants and excavates <cite>Martin-Montalvo13</cite>. COQ7 in humans is targeted to mitochondria, cleaved to become trapped in these organelles, and then function in the biosynthesis of ubiquinone, a cofactor in the electron transport chain. The uncleaved form of these proteins in the nucleus <cite>Monaghan15</cite>.
-==== Subcellular localization ====
+=== Subcellular localization ===
-Human PPTC7 is reported as a mitochondrial protein by multiple datasets summarized in [[http://mitominer.mrc-mbu.cam.ac.uk/release-3.1/report.do?id=1017435&trail=|1017435 Mitominer]], and knockdown in human cells has an effect on mitochondrial respiration <cite>Lanning</cite>, though PPTC7 lacks a mitochondrial targeting signal  [http://www.cbs.dtu.dk/services/TargetP/ TargetP 1.1].
+Human PPTC7 is reported as a mitochondrial protein by multiple datasets summarized in [http://mitominer.mrc-mbu.cam.ac.uk/release-3.1/report.do?id=1017435&trail=|1017435 Mitominer], and knockdown in human cells has an effect on mitochondrial respiration <cite>Lanning</cite>, though PPTC7 lacks a mitochondrial targeting signal  [http://www.cbs.dtu.dk/services/TargetP/ TargetP 1.1].
 === References ===

@@ Line 11: / Line 11: @@
 ==== Subcellular localization ====
-Human PPTC7 is reported as a mitochondrial protein by multiple datasets summarized in [[ Mitominer]], and knockdown in human cells has an effect on mitochondrial respiration <cite>Lanning</cite>, though PPTC lacks a mitochondria targeting signal  [http://www.cbs.dtu.dk/services/TargetP/ TargetP 1.1]. Because COQ7 is localized in both mitochondria and nucleus, further studies need to clarify whether PTC7 is localized to nucleus and dephosphorylates COQ7 in nucleus.
+Human PPTC7 is reported as a mitochondrial protein by multiple datasets summarized in [[http://mitominer.mrc-mbu.cam.ac.uk/release-3.1/report.do?id=1017435&trail=|1017435 Mitominer]], and knockdown in human cells has an effect on mitochondrial respiration <cite>Lanning</cite>, though PPTC lacks a mitochondria targeting signal  [http://www.cbs.dtu.dk/services/TargetP/ TargetP 1.1]. Because COQ7 is localized in both mitochondria and nucleus, further studies need to clarify whether PTC7 is localized to nucleus and dephosphorylates COQ7 in nucleus.
 === References ===