Phosphatase Subfamily PTPRK - Revision history

Gerard: /* Domain Structure */

2016-11-10T02:30:46Z

‎Domain Structure

Gerard at 17:51, 10 September 2016

2016-09-10T17:51:12Z

Gerard: /* PTPRU (PTP-RO/hPTP-J/PTP pi/PTP lambda) */

2016-09-10T17:48:24Z

‎PTPRU (PTP-RO/hPTP-J/PTP pi/PTP lambda)

Gerard: /* PTPRU (PTP-RO/hPTP-J/PTP pi/PTP lambda) */

2016-09-09T23:06:47Z

‎PTPRU (PTP-RO/hPTP-J/PTP pi/PTP lambda)

Gerard at 23:06, 9 September 2016

2016-09-09T23:06:17Z

Gerard: /* Evolution */

2016-09-09T23:05:35Z

‎Evolution

Gerard at 05:16, 13 March 2016

2016-03-13T05:16:24Z

Gerard at 02:08, 11 March 2016

2016-03-11T02:08:18Z

Gerard at 21:35, 6 March 2016

2016-03-06T21:35:32Z

Gerard: /* PTPRU (PTP-RO/hPTP-J/PTP pi/PTP lambda) */

2016-03-06T20:08:15Z

‎PTPRU (PTP-RO/hPTP-J/PTP pi/PTP lambda)

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 ===Domain Structure===
-PTPRK have dual intracellular catalytic domains and an extracellular region consisting of a MAM domain, 1 Ig domain and 4 FN3 domains <cite>Zondag95, Crossland96, Gebbink91</cite>. The MAM domain is essential for homophilic cell-cell interaction and helps determine the specificity of these interactions. Truncated PTPRM is properly expressed at the cell surface but fails to promote cell-cell adhesion. Homophilic cell adhesion is fully restored in a chimeric PTPRM molecule containing the MAM domain of PTPRK. However, this chimeric RPTPM does not interact with either PTPRK or PTPRM <cite>Zondag95</cite>. The Ig domain of PTPRM contains the homophilic binding site and regulates subcellular localization <cite>Vecchio05</cite>.
+PTPRK have dual intracellular catalytic domains and an extracellular region consisting of a MAM domain, 1 Ig domain and 4 FN3 domains <cite>Zondag95, Crossland96, Gebbink91</cite>. The MAM domain is essential for homophilic cell-cell interaction and helps determine the specificity of these interactions. Truncated PTPRM is properly expressed at the cell surface but fails to promote cell-cell adhesion. Homophilic cell adhesion is fully restored in a chimeric PTPRM molecule containing the MAM domain of PTPRK. However, this chimeric protein does not interact with either PTPRK or PTPRM <cite>Zondag95</cite>. The Ig domain of PTPRM contains the homophilic binding site and regulates subcellular localization <cite>Vecchio05</cite>.
 ===Functions===

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 Weak PTPRU expression was detected in peripheral blood lymphocytes, thymus, and spleen even though gene expression was relatively high in the Jurkat T lymphoma cell line. Moreover, PTPRU gene expression was down-regulated after [http://en.wikipedia.org/wiki/Jurkat_cells Jurkat cells] (an immortalized line of human T lymphocyte cells) were stimulated by either Phorbol myristate acetage (PMA) or calcium ionophore <cite>Wang97, Wang99</cite>.
-Both catalytic domains of PTPRU have substitutions that predict inactivity - the D1 domain has a D->E change in the WPD motif and a Y->E in the KNRY motif, while the D2 domain has E->A and F->S at these motifs. However, both domains have been reported to have in vitro catalytic activity <cite></cite>, and mutation of the catalytic cysteines in both domains
+Both catalytic domains of PTPRU have substitutions that predict inactivity - the D1 domain has a D->E change in the WPD motif and a Y->E in the KNRY motif, while the D2 domain has E->A and F->S at these motifs. However, both domains have been reported to have in vitro catalytic activity <cite>Crossland96</cite>, and mutation of the catalytic cysteines in both domains blocks PTPRU ability to block beta catenin activation <cite>Yan06</cite>.
 === External links ===
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 #Zondag95 pmid=7782276
 #Wang96 pmid=8700514
 #Crossland96 pmid=8870675
 #Zhang98 pmid=9722959
 #Novellino08 pmid=16263724

← Older revision		Revision as of 17:48, 10 September 2016
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	Weak PTPRU expression was detected in peripheral blood lymphocytes, thymus, and spleen even though gene expression was relatively high in the Jurkat T lymphoma cell line. Moreover, PTPRU gene expression was down-regulated after [http://en.wikipedia.org/wiki/Jurkat_cells Jurkat cells] (an immortalized line of human T lymphocyte cells) were stimulated by either Phorbol myristate acetage (PMA) or calcium ionophore <cite>Wang97, Wang99</cite>.		Weak PTPRU expression was detected in peripheral blood lymphocytes, thymus, and spleen even though gene expression was relatively high in the Jurkat T lymphoma cell line. Moreover, PTPRU gene expression was down-regulated after [http://en.wikipedia.org/wiki/Jurkat_cells Jurkat cells] (an immortalized line of human T lymphocyte cells) were stimulated by either Phorbol myristate acetage (PMA) or calcium ionophore <cite>Wang97, Wang99</cite>.
		+
		+	Both catalytic domains of PTPRU have substitutions that predict inactivity - the D1 domain has a D->E change in the WPD motif and a Y->E in the KNRY motif, while the D2 domain has E->A and F->S at these motifs. However, both domains have been reported to have in vitro catalytic activity <cite></cite>, and mutation of the catalytic cysteines in both domains

	=== External links ===		=== External links ===

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 ===== PTPRU (PTP-RO/hPTP-J/PTP pi/PTP lambda)=====
-PTPRU is predominantly found in adult brain, lung, and kidney <cite>Cheng97, Sommer97</cite>. It localizes to the adherens junctions with cell adhesion molecules like beta-catenin and E-cadherin <cite>Wang96</cite>. PTPRU directly binds and dephosphorylates beta-catenin, which is a key molecule involved in both cell adhesion and Wnt signaling pathway <cite>Yan02, Yan06</cite>. PTPRU-deficient mice exhibited hypertension and low [http://en.wikipedia.org/wiki/Glomerulus_%28kidney%29 glomerular] filtration rate <cite>Wharram00</cite>.
+PTPRU is predominantly expressed in adult brain, lung, and kidney <cite>Cheng97, Sommer97</cite>. It localizes to the adherens junctions with cell adhesion molecules like beta-catenin and E-cadherin <cite>Wang96</cite>. PTPRU directly binds and dephosphorylates beta-catenin, which is a key molecule involved in both cell adhesion and Wnt signaling pathway <cite>Yan02, Yan06</cite>. PTPRU-deficient mice exhibited hypertension and low [http://en.wikipedia.org/wiki/Glomerulus_%28kidney%29 glomerular] filtration rate <cite>Wharram00</cite>.
 Weak PTPRU expression was detected in peripheral blood lymphocytes, thymus, and spleen even though gene expression was relatively high in the Jurkat T lymphoma cell line. Moreover, PTPRU gene expression was down-regulated after [http://en.wikipedia.org/wiki/Jurkat_cells Jurkat cells] (an immortalized line of human T lymphocyte cells) were stimulated by either Phorbol myristate acetage (PMA) or calcium ionophore <cite>Wang97, Wang99</cite>.

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 [[Phosphatase classification|Phosphatase Classification]]: [[Phosphatase_Fold_CC1|Fold CC1]]:[[Phosphatase_Superfamily_CC1|Superfamily CC1]]: [[Phosphatase_Family_PTP|Family PTP]]: [[Phosphatase_Subfamily_PTPRK|Subfamily PTPRK]]
-PTPRK is a chordate-specific subfamily that regulates cell-cell adhesion. It is involved in human cancer. It also functions in nervous system.
+PTPRK is a chordate-specific subfamily that regulates cell-cell adhesion. It is involved in human cancer and in the nervous system.
 ===Evolution===

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 ===Evolution===
-PTPRK is found in chordates (see [[Phosphatase_Subfamily_PPPRK_TN|technical notes]]). There are four copies in human, which have similar genomic organization, despite great disparities in gene size due to variations in intron length <cite>Besco04</cite>.
+PTPRK is found in chordates (see [[Phosphatase_Subfamily_PPPRK_TN|technical notes]]). There are four copies in human, which have similar genomic organization, despite great disparities in gene size due to variations in intron length <cite>Besco04</cite>. PTPRK may be orthologous to some invertebrate phosphatases, including the [[Phosphatase_Subfamily_Ptp36E|Ptp36E]] subfamily
 ===Domain Structure===

← Older revision		Revision as of 21:35, 6 March 2016
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−	[[Phosphatase classification\|Phosphatase Classification]]: [[Phosphatase_Fold_CC1\|Fold CC1]]:[[Phosphatase_Superfamily_CC1\|Superfamily CC1]]: [[Phosphatase_Family_PTP\|Family PTP]]: [[Phosphatase_Subfamily_PTPRK\|Subfamily PTPRK]] ~~(R2B)~~	+	[[Phosphatase classification\|Phosphatase Classification]]: [[Phosphatase_Fold_CC1\|Fold CC1]]:[[Phosphatase_Superfamily_CC1\|Superfamily CC1]]: [[Phosphatase_Family_PTP\|Family PTP]]: [[Phosphatase_Subfamily_PTPRK\|Subfamily PTPRK]]

	PTPRK is a vertebrate-specific subfamily that regulates cell-cell adhesion. It is involved in human cancer. It also functions in nervous system.		PTPRK is a vertebrate-specific subfamily that regulates cell-cell adhesion. It is involved in human cancer. It also functions in nervous system.