Phosphatase Subfamily SAC1 - Revision history

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‎Domain

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‎Function

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 === Domain ===
-The SAC1 subfamily has SAC phosphatase domain. Human SAC1 has a C-terminal tail of ~90 amino acid. The C-terminal tail a dual-pass transmembrane region. It also has a KxKxx at C-terminus which mediates its interaction with COPI complex <cite>Rohde03</cite>. Within the phosphatase domain, human SAC1 has a RLNSTSP motif (145-151) interacting with 14-3-3, which binds to COPII complex and serves as a cytosolic adaptor in mediating SAC1 transport in COPII-coated vesicle <cite>Pahuja15</cite>.
+The SAC1 subfamily has SAC phosphatase domain.
 === Function ===

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 === Domain ===
-The SAC1 subfamily has a single structural domain, SAC phosphatase domain. It also has a tail which contains localization signal and motifs interacting with other proteins (e.g. coatomer I complex <cite>Rohde03</cite>).
+The SAC1 subfamily has SAC phosphatase domain. Human SAC1 has a C-terminal tail of ~90 amino acid. The C-terminal tail a dual-pass transmembrane region. It also has a KxKxx at C-terminus which mediates its interaction with COPI complex <cite>Rohde03</cite>. Within the phosphatase domain, human SAC1 has a RLNSTSP motif (145-151) interacting with 14-3-3, which binds to COPII complex and serves as a cytosolic adaptor in mediating SAC1 transport in COPII-coated vesicle <cite>Pahuja15</cite>.
 === Function ===
-The members of the SAC1 subfamily are integral membrane phosphoinositide phosphatases that play evolutionary conserved roles in eukaryotic cell physiology <cite>Nemoto00</cite>. It locates in  the endoplasmic reticulum (ER) and the Golgi apparatus in yeast and mammals.
+The members of the SAC1 subfamily are integral membrane phosphoinositide phosphatases that play evolutionary conserved roles in eukaryotic cell physiology <cite>Nemoto00</cite>. It locates in  the endoplasmic reticulum (ER) and the Golgi apparatus in yeast <cite>Konrad02</cite> and mammals <cite>Rohde03</cite>.
 SAC1 is best characterized in Saccharomyces cerevisiae <cite>Cleves89, Cai14</cite>. Yeast SAC1 mutants display a wide array of phenotypes including inositol auxotrophy, cold sensitivity, secretory defects, disturbed ATP transport into the ER, or suppression of actin gene mutations. Sac1p is able to dephosphorylate PtdIns[3]P, PtdIns[4]P, and PtdIns[3,5]P2 in vitro, the role of Sac1p in processes involving PtdIns[4]P has been the primary focus of in vivo studies (see [http://www.yeastgenome.org/locus/S000001695/overview#paragraph SGD]).
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 #Cai14 pmid=25113029
 #Cleves89 pmid=2687291
 #Liu08 pmid=18480408
 #Nemoto00 pmid=10887188
 #Rohde03 pmid=14527956
 </biblio>

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 The members of the SAC1 subfamily are integral membrane phosphoinositide phosphatases that play evolutionary conserved roles in eukaryotic cell physiology <cite>Nemoto00</cite>. It locates in  the endoplasmic reticulum (ER) and the Golgi apparatus in yeast and mammals.
-SAC1 is best characterized in Saccharomyces cerevisiae <cite>Cleves89, Cai14</cite>. Yeast SAC1 mutants display a wide array of phenotypes including inositol auxotrophy, cold sensitivity, secretory defects, disturbed ATP transport into the ER, or suppression of actin gene mutations.
+SAC1 is best characterized in Saccharomyces cerevisiae <cite>Cleves89, Cai14</cite>. Yeast SAC1 mutants display a wide array of phenotypes including inositol auxotrophy, cold sensitivity, secretory defects, disturbed ATP transport into the ER, or suppression of actin gene mutations. Sac1p is able to dephosphorylate PtdIns[3]P, PtdIns[4]P, and PtdIns[3,5]P2 in vitro, the role of Sac1p in processes involving PtdIns[4]P has been the primary focus of in vivo studies (see [http://www.yeastgenome.org/locus/S000001695/overview#paragraph SGD]).
 In mammals, it regulates Golgi membrane morphology and mitotic spindle organization <cite>Liu08</cite>. The human SAC1 is widely expressed in different tissues (see [http://www.gtexportal.org/home/gene/SACM1L GTEx]). It interacts with the coatomer I complex via a putative COPI interaction motif (KXKXX) at its C terminus in a manner dependent on its catalytic activity <cite>Rohde03</cite>.

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 [[Phosphatase classification|Phosphatase Classification]]: [[Phosphatase_Fold_CC1|Fold CC1]]: [[Phosphatase_Superfamily_CC1|Superfamily CC1]]:  [[Phosphatase_Family_Sac|Family Sac]]: [[Phosphatase_Subfamily_SAC1|Subfamily SAC1]]
-SAC1 is an integral membrane phosphoinositide phosphatase conserved in eukaryotes.
+SAC1 is an integral membrane phosphoinositide phosphatase located in endoplasmic reticulum (ER) and golgi apparatus. SAC1 is conserved in eukaryotes.
 === Evolution ===

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 __NOTOC__
 [[Phosphatase classification|Phosphatase Classification]]: [[Phosphatase_Fold_CC1|Fold CC1]]: [[Phosphatase_Superfamily_CC1|Superfamily CC1]]:  [[Phosphatase_Family_Sac|Family Sac]]: [[Phosphatase_Subfamily_SAC1|Subfamily SAC1]]
 === Evolution ===

← Older revision		Revision as of 17:20, 15 April 2015
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	=== Evolution ===		=== Evolution ===
	The SAC1 subfamily is conserved in eukaryotes. It is present in almost all the 203 eukaryotic genomes in [http://resdev.gene.com/gOrtholog/view/cluster/MC0000957/overview gOrtholog database (internal)].		The SAC1 subfamily is conserved in eukaryotes. It is present in almost all the 203 eukaryotic genomes in [http://resdev.gene.com/gOrtholog/view/cluster/MC0000957/overview gOrtholog database (internal)].
		+
		+	=== Domain ===
		+	The SAC1 subfamily has a single structural domain, SAC phosphatase domain. It also has a tail which contains localization signal and motifs interacting with other proteins (e.g. coatomer I complex <cite>Rohde03</cite>).
		+
		+	=== Function ===
		+	The members of the SAC1 subfamily are integral membrane phosphoinositide phosphatases that play evolutionary conserved roles in eukaryotic cell physiology <cite>Nemoto00</cite>. It locates in the endoplasmic reticulum (ER) and the Golgi apparatus in yeast and mammals.
		+
		+	SAC1 is best characterized in Saccharomyces cerevisiae <cite>Cleves89, Cai14</cite>. Yeast SAC1 mutants display a wide array of phenotypes including inositol auxotrophy, cold sensitivity, secretory defects, disturbed ATP transport into the ER, or suppression of actin gene mutations.
		+
		+	In mammals, it regulates Golgi membrane morphology and mitotic spindle organization <cite>Liu08</cite>. The human SAC1 is widely expressed in different tissues (see [http://www.gtexportal.org/home/gene/SACM1L GTEx]). It interacts with the coatomer I complex via a putative COPI interaction motif (KXKXX) at its C terminus in a manner dependent on its catalytic activity <cite>Rohde03</cite>.
		+
		+	=== References ===
		+	<biblio>
		+	#Cai14 pmid=25113029
		+	#Cleves89 pmid=2687291
		+	#Liu08 pmid=18480408
		+	#Nemoto00 pmid=10887188
		+	#Rohde03 pmid=14527956
		+	</biblio>

← Older revision		Revision as of 16:45, 15 April 2015
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	__NOTOC__		__NOTOC__
	[[Phosphatase classification\|Phosphatase Classification]]: [[Phosphatase_Fold_CC1\|Fold CC1]]: [[Phosphatase_Superfamily_CC1\|Superfamily CC1]]: [[Phosphatase_Family_Sac\|Family Sac]]: [[Phosphatase_Subfamily_SAC1\|Subfamily SAC1]]		[[Phosphatase classification\|Phosphatase Classification]]: [[Phosphatase_Fold_CC1\|Fold CC1]]: [[Phosphatase_Superfamily_CC1\|Superfamily CC1]]: [[Phosphatase_Family_Sac\|Family Sac]]: [[Phosphatase_Subfamily_SAC1\|Subfamily SAC1]]
		+
		+	=== Evolution ===
		+	The SAC1 subfamily is conserved in eukaryotes. It is present in almost all the 203 eukaryotic genomes in [http://resdev.gene.com/gOrtholog/view/cluster/MC0000957/overview gOrtholog database (internal)].

← Older revision		Revision as of 16:41, 15 April 2015
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	__NOTOC__		__NOTOC__
−	[[Phosphatase classification\|Phosphatase Classification]]: [[Phosphatase_Fold_CC1\|Fold CC1]]: [[Phosphatase_Superfamily_CC1\|Superfamily CC1]]: [[Phosphatase_Family_Sac\|Family Sac]]: [[~~Phosphatase_Subamily_SAC1~~\|Subfamily SAC1]]	+	[[Phosphatase classification\|Phosphatase Classification]]: [[Phosphatase_Fold_CC1\|Fold CC1]]: [[Phosphatase_Superfamily_CC1\|Superfamily CC1]]: [[Phosphatase_Family_Sac\|Family Sac]]: [[Phosphatase_Subfamily_SAC1\|Subfamily SAC1]]