Phosphatase Superfamily PHP - Revision history

Mark: /* Evolution */

2015-06-02T17:18:45Z

‎Evolution

Mark: /* Evolution */

2015-06-02T17:16:02Z

‎Evolution

Mark at 01:45, 2 June 2015

2015-06-02T01:45:54Z

Mark at 17:44, 1 January 2015

2015-01-01T17:44:26Z

Gerard at 01:00, 1 May 2014

2014-05-01T01:00:28Z

Gerard: Created page with "NOTOC Phosphatase Classification: PHP Superfamily PHP is the only known protein Histidine phosphates, and ..."

2014-05-01T01:00:12Z

Created page with "__NOTOC__ Phosphatase Classification: PHP Superfamily PHP is the only known protein Histidine phosphates, and ..."

New page

__NOTOC__

[[Phosphatase classification|Phosphatase Classification]]: [[Phosphatase_Superfamily_PHP|PHP Superfamily]]

PHP is the only known protein Histidine phosphates, and consists of a single family, also known as [[Phosphatase_Family_PHP|PHP]].

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 === Evolution ===
-PHP is found throughout eukaryotes, though lost from fungi. It is usually single copy per genome, but four are found in ''Drosophila melanogaster''. Three of them (janA, janB, con) arranged as tandem duplications which are conserved other flies of melanogaster group. The janA is canonical PHP, but the other three have substitution at conserved positions  of canonical PHPs (memo: carry out positive selection analysis to infer whether they evolved different functions). They The most conserved positions include:
+PHP is found throughout eukaryotes, though lost from fungi. It is usually single copy per genome, but four are found in ''Drosophila melanogaster''. Three of them (janA, janB, con) arranged as tandem duplications which are conserved other flies of melanogaster group. The janA is canonical PHP, but the other three have substitution at conserved positions  of canonical PHPs (memo: carry out positive selection analysis to infer whether they evolved different functions). The most conserved positions include:
 * Motif 1: 18 GxFKY 22
 * Motif 2: 45 RG 46

@@ Line 5: / Line 5: @@
 === Evolution ===
-PHP is found throughout eukaryotes, though lost from fungi. It is usually single copy per genome, but four are found in fruit fly. The most conserved positions include:
+PHP is found throughout eukaryotes, though lost from fungi. It is usually single copy per genome, but four are found in ''Drosophila melanogaster''. Three of them (janA, janB, con) arranged as tandem duplications which are conserved other flies of melanogaster group. The janA is canonical PHP, but the other three have substitution at conserved positions  of canonical PHPs (memo: carry out positive selection analysis to infer whether they evolved different functions). They The most conserved positions include:
 * Motif 1: 18 GxFKY 22
 * Motif 2: 45 RG 46

@@ Line 5: / Line 5: @@
 === Evolution ===
-PHP is found throughout eukaryotes, though lost from fungi. It is usually single copy per genome, but four are found in fruit fly.
+PHP is found throughout eukaryotes, though lost from fungi. It is usually single copy per genome, but four are found in fruit fly. The most conserved positions include:
 === Domain ===
-PHP has a single domain: catalytic domain. The structure of the domain has been solved and a potential enzymatic mechanism proposed <cite>Gong</cite>.
+PHP has a single domain: catalytic domain. The structure of the domain has been solved and a potential enzymatic mechanism proposed (see figure 4 in <cite>Gong</cite>): (i) the substrate binds to the protein by forming four H-bonds; (ii) His-53 acts as a general base to activate a water molecule, which in turn functions as a nucleophile to attack the phosphate substrate; and (iii) the positively charged amine group of Lys-21 helps in stabilizing the transition state during catalysis.
 === Functions ===
 Several substrates have been reported, including ''beta'' subunit of heterotrimeric G proteins <cite>Maurer04</cite>, the metabolic enzyme adenosine 5’-triphosphate-citrate lyase (ACL) <cite>Klumpp03</cite>, and the Ca2+-activated K+ channel KCa3.1 <cite>Skolnik08</cite>. These are known or suspected substrates of the nucleoside diphosphate kinases ([http://kinase.com/wiki/index.php/Kinase_Group_NDK NDK]).
-Its role in neuronal cells is particularly interesting. In ''C. elegans'', the ortholog is expressed exclusively in neurons <cite>Klumpp02</cite>. In human cells, the overexpression of PHPT1 decreases the activity of adenosine 5’-triphosphate-citrate lyase (ACL) and reduces the viability of neuronal cells <cite>Klumpp09</cite>.
+Its role in neuronal cells is particularly interesting. Human PHP (PHPT1) is widely expressed across different tissues, most abundantly in cerebellum and pituitary, according to RNA-seq data from [http://www.gtexportal.org/home/gene/PHPT1 GTEx]. In ''C. elegans'', PHP is expressed exclusively in neurons <cite>Klumpp02</cite>. In human cells, the overexpression of PHPT1 decreases the activity of adenosine 5’-triphosphate-citrate lyase (ACL) and reduces the viability of neuronal cells <cite>Klumpp09</cite>.
-−
 === References ===

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 __NOTOC__
-[[Phosphatase classification|Phosphatase Classification]]: [[Phosphatase_Superfamily_PHP|PHP Superfamily]]
+PHP is the only phosphatase known to be histidine-specific.
-PHP is the only known protein Histidine phosphate, and consists of a single family, also known as [[Phosphatase_Family_PHP|PHP]].
+=== Evolution ===

← Older revision		Revision as of 01:00, 1 May 2014
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	[[Phosphatase classification\|Phosphatase Classification]]: [[Phosphatase_Superfamily_PHP\|PHP Superfamily]]		[[Phosphatase classification\|Phosphatase Classification]]: [[Phosphatase_Superfamily_PHP\|PHP Superfamily]]

−	PHP is the only known protein Histidine ~~phosphates~~, and consists of a single family, also known as [[Phosphatase_Family_PHP\|PHP]].	+	PHP is the only known protein Histidine phosphate, and consists of a single family, also known as [[Phosphatase_Family_PHP\|PHP]].

Phosphatase Superfamily PHP - Revision history

Mark: /* Evolution */

Mark: /* Evolution */

Mark at 01:45, 2 June 2015

Mark at 17:44, 1 January 2015

Gerard at 01:00, 1 May 2014

Gerard: Created page with "__NOTOC__ Phosphatase Classification: PHP Superfamily PHP is the only known protein Histidine phosphates, and ..."

Gerard: Created page with "NOTOC Phosphatase Classification: PHP Superfamily PHP is the only known protein Histidine phosphates, and ..."